Refractometric methods for determining total protein
نویسندگان
چکیده
منابع مشابه
Three turbidimetric methods for determining total protein compared.
We used human serum protein fractions to evaluate the sensitivity and bias of three turbidimetric methods for determining concentrations of proteins. Each fraction (Cohn Fractions II, III, IV, and V) was assigned a protein concentration value that was determined by the biuret method, which we calibrated with purified monomer of human serum albumin. All three turbidimetric methods (those involvi...
متن کاملFour methods for determining total protein compared by using purified protein fractions from human serum.
The proportional bias of four methods frequently used for determining low concentrations of protein was evaluated with human serum protein fractions (Cohn Fractions II, III, IV, and V). Each fraction was assigned a protein concentration value as determined by the biuret method, calibrated with purified human serum albumin monomer. All four methods (Coomassie Brilliant Blue dye-binding, the meth...
متن کاملNew refractometric methods for the determination of total proteins in serum and in urine.
New, precise, physical methods for the determination of total protein in serum or plasma and in urine are described. They are based upon the refractometric determination of total solids in these fluids before and after protein has been removed by coagulation and, in ordinary use, they require no special standardization or calibration. The urine method is applicable primarily to protein concentr...
متن کاملSix methods for determining urinary protein compared.
Inter-laboratory surveys have shown that "routine methods" of urinary protein determination are often unsatisfactory. Therefore, we compared six frequently used methods for determination of protein in urine with respect to linearity, within-batch and between-batch precision, comparative bias, and practicability. We assayed dilutions of human and bovine albumin and serum, and fresh and lyophiliz...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1937
ISSN: 0306-3283
DOI: 10.1042/bj0310205